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itsn1 flag construct  (Addgene inc)


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    Structured Review

    Addgene inc itsn1 flag construct
    Itsn1 Flag Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flag+itsn1/Flag+Intersectin+Long+(human)+(Plasmid+%2347393)/pmc12124131-443-2-5
    Average 93 stars, based on 2 article reviews
    itsn1 flag construct - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: A DNM2 Centronuclear Myopathy Mutation Reveals a Link between Recycling Endosome Scission and Autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti AP2 Thermofisher Cat#(MA1-064) AB_2258307 rabbit anti-ATG3 ABCAM Cat#(ab108251) AB_10865145 rabbit anti-ATG9A ABCAM Cat#(ab108338) AB_10863880 rabbit anti-ATG9A Eurogentec rabbit 1963 (This paper) rabbit anti-ATG16L1 Cell Signaling Cat#(D6D5) AB_10950320 mouse anti-Cathepsin D ABCAM Cat#(ab6313) AB_305416 rabbit anti DNM2 ABCAM Cat#(ab65556) AB_1603781 mouse anti-Flag SIGMA Cat#(F1804) AB_262044 rabbit anti-GABARAP GENERON Cat#(AP1821A) AB_2278762 rabbit anti-GABARAP-L1 Proteintech Cat#(110101) AB_2294415 GABARAP-L2 MBL Cat#(PM038) AB_843130 mouse anti-GAPDH ABCAM Cat#(ab8245) AB_2107448 rabbit anti-GFP Invitrogen Cat#(A6455) AB_221570 mouse anti-HA Biolegend Cat#(16B12) AB_10063630 rabbit anti-ITSN1 Millipore Cat#(ABN1378) mouse anti-LC3B Nanotools Cat#(0231-100/LC3-5F10) AB_2722733 rabbit anti-LC3B ABCAM Cat#(ab192890) rabbit anti-P62 MBL Cat#(PM045) AB_1279301 mouse anti-Myc tag ABCAM ab18185 AB_444307 rabbit anti-RAB11A ABCAM Cat# (ab128913) AB_11140633 Chemicals, Peptides, and Recombinant Proteins Ferrofluid EMG508 Megatech ltd. Cat# (TSD-06-24-11-1) Bafilomycin A1 Enzo Cat# (BML-CM110) SMER28 Tocris Cat# (4297) Rapamycin LC Laboratories Cat# (R-5000) HaloTag TMR ligand Promega Cat# (G825A) HaloTag Alexa 488 ligand Promega Cat# (G100A) Seahorse XF Plasma membrane permeabilizer Agilent Technologies Cat# (102504-100) DNM2 recombinant protein Origene, Cat# (TP323585) LC3 recombinant protein This paper N/A (Continued on next page) Developmental Cell 53, 154–168.e1–e6, April 20, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays GFP-TRAP Chromotek Cat# (Gtma-20) Myc-TRAP Chromotek Cat# (ytma-20) Anti-HA Magnetic Beads Pierce Cat# (88836) Experimental Models: Cell Lines U2OS Halotag-LC3 This paper N/A HeLa DNM2-PEGFP WT This paper N/A HeLa DNM2-PEGFP R465W This paper N/A HeLa DNM2-PEGFP W525L This paper N/A RFP-GFP tandem fluorescent-tagged LC3 Ravikumar et al., 2010 N/A Mouse embryonic fibroblasts DNM2R465W Durieux et al., 2010 N/A Mouse embryonic fibroblasts WT Durieux et al., 2010 N/A Patient-derived fibroblasts DNM2 R465W Trochet et al., 2018 N/A Control human fibroblasts C1 Trochet et al., 2018 N/A Control human fibroblasts 711 Coriell Institute GM04711 Control human fibroblasts 729 Coriell Institute GM04729 Control human fibroblasts 865 Coriell Institute GM04865 Oligonucleotides (primers) Primer for DNM2 R465W: TCA CCA CCT ACA TCT GGG AGC GAG AAG GG This paper N/A Primer for DNM2 W525L: TGA TCC GCA GGG GCT TGT TGA CCA TCA ACA AC This paper N/A Primer for LC3 K51A: CCG TCC TGG ACA AGA CCG CGT TCC TTG TAC CTG ATC This paper N/A Primer for LC3 R70A: CAT CAA GAT AAT TAG AAG GGC CCT GCA GCT CAA TGC TAA C This paper N/A For siRNA sequences, see Table S1 Recombinant DNA pEGFP-RAB11A Puri et al., 2013 N/A mCherry-RAB11A Puri et al., 2018a N/A pEGFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A pECFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A RFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A LC3-PGEX-6P-1 Mineyuki Mizuguchi (Toyama University, Japan) N/A DNM2-WT-PEGFP Mark McNiven (Mayo Clinic Minnesota, USA) N/A HA -DNM2-WT Addgene Cat# (34684) Flag-ITSN1-short Addgene Cat# (47392) pEGFP-ITSN1 short Addgene Cat# (47394) Flag-empty vector SIGMA Cat# (E4901) Myc-SNX18-WT Anne Simonsen (University of Oslo, Norway) N/A Myc-SNX18- W38F Anne Simonsen (University of Oslo, Norway) N/A httQ74-HA Korolchuk et al., 2009 N/A Software and Algorithms Prism 7 GraphPad N/A ZEN Black Carl Zeiss Microscopy N/A Volocity PerkinElmer N/A Image Studio Lite LI-COR, Inc N/A ImageJ National Institute of Health, USA N/A e2 Developmental Cell 53, 154–168.e1–e6, April 20, 2020 ..

    Control:

    Article Title: A DNM2 Centronuclear Myopathy Mutation Reveals a Link between Recycling Endosome Scission and Autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti AP2 Thermofisher Cat#(MA1-064) AB_2258307 rabbit anti-ATG3 ABCAM Cat#(ab108251) AB_10865145 rabbit anti-ATG9A ABCAM Cat#(ab108338) AB_10863880 rabbit anti-ATG9A Eurogentec rabbit 1963 (This paper) rabbit anti-ATG16L1 Cell Signaling Cat#(D6D5) AB_10950320 mouse anti-Cathepsin D ABCAM Cat#(ab6313) AB_305416 rabbit anti DNM2 ABCAM Cat#(ab65556) AB_1603781 mouse anti-Flag SIGMA Cat#(F1804) AB_262044 rabbit anti-GABARAP GENERON Cat#(AP1821A) AB_2278762 rabbit anti-GABARAP-L1 Proteintech Cat#(110101) AB_2294415 GABARAP-L2 MBL Cat#(PM038) AB_843130 mouse anti-GAPDH ABCAM Cat#(ab8245) AB_2107448 rabbit anti-GFP Invitrogen Cat#(A6455) AB_221570 mouse anti-HA Biolegend Cat#(16B12) AB_10063630 rabbit anti-ITSN1 Millipore Cat#(ABN1378) mouse anti-LC3B Nanotools Cat#(0231-100/LC3-5F10) AB_2722733 rabbit anti-LC3B ABCAM Cat#(ab192890) rabbit anti-P62 MBL Cat#(PM045) AB_1279301 mouse anti-Myc tag ABCAM ab18185 AB_444307 rabbit anti-RAB11A ABCAM Cat# (ab128913) AB_11140633 Chemicals, Peptides, and Recombinant Proteins Ferrofluid EMG508 Megatech ltd. Cat# (TSD-06-24-11-1) Bafilomycin A1 Enzo Cat# (BML-CM110) SMER28 Tocris Cat# (4297) Rapamycin LC Laboratories Cat# (R-5000) HaloTag TMR ligand Promega Cat# (G825A) HaloTag Alexa 488 ligand Promega Cat# (G100A) Seahorse XF Plasma membrane permeabilizer Agilent Technologies Cat# (102504-100) DNM2 recombinant protein Origene, Cat# (TP323585) LC3 recombinant protein This paper N/A (Continued on next page) Developmental Cell 53, 154–168.e1–e6, April 20, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays GFP-TRAP Chromotek Cat# (Gtma-20) Myc-TRAP Chromotek Cat# (ytma-20) Anti-HA Magnetic Beads Pierce Cat# (88836) Experimental Models: Cell Lines U2OS Halotag-LC3 This paper N/A HeLa DNM2-PEGFP WT This paper N/A HeLa DNM2-PEGFP R465W This paper N/A HeLa DNM2-PEGFP W525L This paper N/A RFP-GFP tandem fluorescent-tagged LC3 Ravikumar et al., 2010 N/A Mouse embryonic fibroblasts DNM2R465W Durieux et al., 2010 N/A Mouse embryonic fibroblasts WT Durieux et al., 2010 N/A Patient-derived fibroblasts DNM2 R465W Trochet et al., 2018 N/A Control human fibroblasts C1 Trochet et al., 2018 N/A Control human fibroblasts 711 Coriell Institute GM04711 Control human fibroblasts 729 Coriell Institute GM04729 Control human fibroblasts 865 Coriell Institute GM04865 Oligonucleotides (primers) Primer for DNM2 R465W: TCA CCA CCT ACA TCT GGG AGC GAG AAG GG This paper N/A Primer for DNM2 W525L: TGA TCC GCA GGG GCT TGT TGA CCA TCA ACA AC This paper N/A Primer for LC3 K51A: CCG TCC TGG ACA AGA CCG CGT TCC TTG TAC CTG ATC This paper N/A Primer for LC3 R70A: CAT CAA GAT AAT TAG AAG GGC CCT GCA GCT CAA TGC TAA C This paper N/A For siRNA sequences, see Table S1 Recombinant DNA pEGFP-RAB11A Puri et al., 2013 N/A mCherry-RAB11A Puri et al., 2018a N/A pEGFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A pECFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A RFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A LC3-PGEX-6P-1 Mineyuki Mizuguchi (Toyama University, Japan) N/A DNM2-WT-PEGFP Mark McNiven (Mayo Clinic Minnesota, USA) N/A HA -DNM2-WT Addgene Cat# (34684) Flag-ITSN1-short Addgene Cat# (47392) pEGFP-ITSN1 short Addgene Cat# (47394) Flag-empty vector SIGMA Cat# (E4901) Myc-SNX18-WT Anne Simonsen (University of Oslo, Norway) N/A Myc-SNX18- W38F Anne Simonsen (University of Oslo, Norway) N/A httQ74-HA Korolchuk et al., 2009 N/A Software and Algorithms Prism 7 GraphPad N/A ZEN Black Carl Zeiss Microscopy N/A Volocity PerkinElmer N/A Image Studio Lite LI-COR, Inc N/A ImageJ National Institute of Health, USA N/A e2 Developmental Cell 53, 154–168.e1–e6, April 20, 2020 ..

    Recombinant:

    Article Title: A DNM2 Centronuclear Myopathy Mutation Reveals a Link between Recycling Endosome Scission and Autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti AP2 Thermofisher Cat#(MA1-064) AB_2258307 rabbit anti-ATG3 ABCAM Cat#(ab108251) AB_10865145 rabbit anti-ATG9A ABCAM Cat#(ab108338) AB_10863880 rabbit anti-ATG9A Eurogentec rabbit 1963 (This paper) rabbit anti-ATG16L1 Cell Signaling Cat#(D6D5) AB_10950320 mouse anti-Cathepsin D ABCAM Cat#(ab6313) AB_305416 rabbit anti DNM2 ABCAM Cat#(ab65556) AB_1603781 mouse anti-Flag SIGMA Cat#(F1804) AB_262044 rabbit anti-GABARAP GENERON Cat#(AP1821A) AB_2278762 rabbit anti-GABARAP-L1 Proteintech Cat#(110101) AB_2294415 GABARAP-L2 MBL Cat#(PM038) AB_843130 mouse anti-GAPDH ABCAM Cat#(ab8245) AB_2107448 rabbit anti-GFP Invitrogen Cat#(A6455) AB_221570 mouse anti-HA Biolegend Cat#(16B12) AB_10063630 rabbit anti-ITSN1 Millipore Cat#(ABN1378) mouse anti-LC3B Nanotools Cat#(0231-100/LC3-5F10) AB_2722733 rabbit anti-LC3B ABCAM Cat#(ab192890) rabbit anti-P62 MBL Cat#(PM045) AB_1279301 mouse anti-Myc tag ABCAM ab18185 AB_444307 rabbit anti-RAB11A ABCAM Cat# (ab128913) AB_11140633 Chemicals, Peptides, and Recombinant Proteins Ferrofluid EMG508 Megatech ltd. Cat# (TSD-06-24-11-1) Bafilomycin A1 Enzo Cat# (BML-CM110) SMER28 Tocris Cat# (4297) Rapamycin LC Laboratories Cat# (R-5000) HaloTag TMR ligand Promega Cat# (G825A) HaloTag Alexa 488 ligand Promega Cat# (G100A) Seahorse XF Plasma membrane permeabilizer Agilent Technologies Cat# (102504-100) DNM2 recombinant protein Origene, Cat# (TP323585) LC3 recombinant protein This paper N/A (Continued on next page) Developmental Cell 53, 154–168.e1–e6, April 20, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays GFP-TRAP Chromotek Cat# (Gtma-20) Myc-TRAP Chromotek Cat# (ytma-20) Anti-HA Magnetic Beads Pierce Cat# (88836) Experimental Models: Cell Lines U2OS Halotag-LC3 This paper N/A HeLa DNM2-PEGFP WT This paper N/A HeLa DNM2-PEGFP R465W This paper N/A HeLa DNM2-PEGFP W525L This paper N/A RFP-GFP tandem fluorescent-tagged LC3 Ravikumar et al., 2010 N/A Mouse embryonic fibroblasts DNM2R465W Durieux et al., 2010 N/A Mouse embryonic fibroblasts WT Durieux et al., 2010 N/A Patient-derived fibroblasts DNM2 R465W Trochet et al., 2018 N/A Control human fibroblasts C1 Trochet et al., 2018 N/A Control human fibroblasts 711 Coriell Institute GM04711 Control human fibroblasts 729 Coriell Institute GM04729 Control human fibroblasts 865 Coriell Institute GM04865 Oligonucleotides (primers) Primer for DNM2 R465W: TCA CCA CCT ACA TCT GGG AGC GAG AAG GG This paper N/A Primer for DNM2 W525L: TGA TCC GCA GGG GCT TGT TGA CCA TCA ACA AC This paper N/A Primer for LC3 K51A: CCG TCC TGG ACA AGA CCG CGT TCC TTG TAC CTG ATC This paper N/A Primer for LC3 R70A: CAT CAA GAT AAT TAG AAG GGC CCT GCA GCT CAA TGC TAA C This paper N/A For siRNA sequences, see Table S1 Recombinant DNA pEGFP-RAB11A Puri et al., 2013 N/A mCherry-RAB11A Puri et al., 2018a N/A pEGFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A pECFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A RFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A LC3-PGEX-6P-1 Mineyuki Mizuguchi (Toyama University, Japan) N/A DNM2-WT-PEGFP Mark McNiven (Mayo Clinic Minnesota, USA) N/A HA -DNM2-WT Addgene Cat# (34684) Flag-ITSN1-short Addgene Cat# (47392) pEGFP-ITSN1 short Addgene Cat# (47394) Flag-empty vector SIGMA Cat# (E4901) Myc-SNX18-WT Anne Simonsen (University of Oslo, Norway) N/A Myc-SNX18- W38F Anne Simonsen (University of Oslo, Norway) N/A httQ74-HA Korolchuk et al., 2009 N/A Software and Algorithms Prism 7 GraphPad N/A ZEN Black Carl Zeiss Microscopy N/A Volocity PerkinElmer N/A Image Studio Lite LI-COR, Inc N/A ImageJ National Institute of Health, USA N/A e2 Developmental Cell 53, 154–168.e1–e6, April 20, 2020 ..

    Software:

    Article Title: A DNM2 Centronuclear Myopathy Mutation Reveals a Link between Recycling Endosome Scission and Autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti AP2 Thermofisher Cat#(MA1-064) AB_2258307 rabbit anti-ATG3 ABCAM Cat#(ab108251) AB_10865145 rabbit anti-ATG9A ABCAM Cat#(ab108338) AB_10863880 rabbit anti-ATG9A Eurogentec rabbit 1963 (This paper) rabbit anti-ATG16L1 Cell Signaling Cat#(D6D5) AB_10950320 mouse anti-Cathepsin D ABCAM Cat#(ab6313) AB_305416 rabbit anti DNM2 ABCAM Cat#(ab65556) AB_1603781 mouse anti-Flag SIGMA Cat#(F1804) AB_262044 rabbit anti-GABARAP GENERON Cat#(AP1821A) AB_2278762 rabbit anti-GABARAP-L1 Proteintech Cat#(110101) AB_2294415 GABARAP-L2 MBL Cat#(PM038) AB_843130 mouse anti-GAPDH ABCAM Cat#(ab8245) AB_2107448 rabbit anti-GFP Invitrogen Cat#(A6455) AB_221570 mouse anti-HA Biolegend Cat#(16B12) AB_10063630 rabbit anti-ITSN1 Millipore Cat#(ABN1378) mouse anti-LC3B Nanotools Cat#(0231-100/LC3-5F10) AB_2722733 rabbit anti-LC3B ABCAM Cat#(ab192890) rabbit anti-P62 MBL Cat#(PM045) AB_1279301 mouse anti-Myc tag ABCAM ab18185 AB_444307 rabbit anti-RAB11A ABCAM Cat# (ab128913) AB_11140633 Chemicals, Peptides, and Recombinant Proteins Ferrofluid EMG508 Megatech ltd. Cat# (TSD-06-24-11-1) Bafilomycin A1 Enzo Cat# (BML-CM110) SMER28 Tocris Cat# (4297) Rapamycin LC Laboratories Cat# (R-5000) HaloTag TMR ligand Promega Cat# (G825A) HaloTag Alexa 488 ligand Promega Cat# (G100A) Seahorse XF Plasma membrane permeabilizer Agilent Technologies Cat# (102504-100) DNM2 recombinant protein Origene, Cat# (TP323585) LC3 recombinant protein This paper N/A (Continued on next page) Developmental Cell 53, 154–168.e1–e6, April 20, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays GFP-TRAP Chromotek Cat# (Gtma-20) Myc-TRAP Chromotek Cat# (ytma-20) Anti-HA Magnetic Beads Pierce Cat# (88836) Experimental Models: Cell Lines U2OS Halotag-LC3 This paper N/A HeLa DNM2-PEGFP WT This paper N/A HeLa DNM2-PEGFP R465W This paper N/A HeLa DNM2-PEGFP W525L This paper N/A RFP-GFP tandem fluorescent-tagged LC3 Ravikumar et al., 2010 N/A Mouse embryonic fibroblasts DNM2R465W Durieux et al., 2010 N/A Mouse embryonic fibroblasts WT Durieux et al., 2010 N/A Patient-derived fibroblasts DNM2 R465W Trochet et al., 2018 N/A Control human fibroblasts C1 Trochet et al., 2018 N/A Control human fibroblasts 711 Coriell Institute GM04711 Control human fibroblasts 729 Coriell Institute GM04729 Control human fibroblasts 865 Coriell Institute GM04865 Oligonucleotides (primers) Primer for DNM2 R465W: TCA CCA CCT ACA TCT GGG AGC GAG AAG GG This paper N/A Primer for DNM2 W525L: TGA TCC GCA GGG GCT TGT TGA CCA TCA ACA AC This paper N/A Primer for LC3 K51A: CCG TCC TGG ACA AGA CCG CGT TCC TTG TAC CTG ATC This paper N/A Primer for LC3 R70A: CAT CAA GAT AAT TAG AAG GGC CCT GCA GCT CAA TGC TAA C This paper N/A For siRNA sequences, see Table S1 Recombinant DNA pEGFP-RAB11A Puri et al., 2013 N/A mCherry-RAB11A Puri et al., 2018a N/A pEGFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A pECFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A RFP-LC3 Tamotsu Yoshimori (Osaka University, Japan) N/A LC3-PGEX-6P-1 Mineyuki Mizuguchi (Toyama University, Japan) N/A DNM2-WT-PEGFP Mark McNiven (Mayo Clinic Minnesota, USA) N/A HA -DNM2-WT Addgene Cat# (34684) Flag-ITSN1-short Addgene Cat# (47392) pEGFP-ITSN1 short Addgene Cat# (47394) Flag-empty vector SIGMA Cat# (E4901) Myc-SNX18-WT Anne Simonsen (University of Oslo, Norway) N/A Myc-SNX18- W38F Anne Simonsen (University of Oslo, Norway) N/A httQ74-HA Korolchuk et al., 2009 N/A Software and Algorithms Prism 7 GraphPad N/A ZEN Black Carl Zeiss Microscopy N/A Volocity PerkinElmer N/A Image Studio Lite LI-COR, Inc N/A ImageJ National Institute of Health, USA N/A e2 Developmental Cell 53, 154–168.e1–e6, April 20, 2020 ..



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